CA12 anticorps (AA 45-298)

N° du produit ABIN7601765

Cet anticorps anti-CA12 est un anticorps Lapin Polyclonal détectant CA12 dans WB et ELISA. Adapté pour Humain.

Aperçu rapide pour CA12 anticorps (AA 45-298) (ABIN7601765)

Antigène: CA12 (Carbonic Anhydrase 12 (CA12))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CA12 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-CA12 anticorps

Épitope: AA 45-298

Fonction: Anti-CA12 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-CA12 Antibody Picoband® (ABIN7601765). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human CA12 recombinant protein (Position: K45-A298).

Isotype: IgG

Information d'application

Indications d'application: "Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
"1. Hong, JH, Muhammad, E, Zheng, C, Hershkovitz, E, Alkrinawi, S, Loewenthal, N, Parvari, R, Muallem, S (15 December 2015). "Essential role of carbonic anhydrase XII in secretory gland fluid and HCO3 (-) secretion revealed by disease causing human mutation". The Journal of Physiology. 593 (24): 5299-312. 2. Lee, M, Vecchio-Pagán, B, Sharma, N, Waheed, A, Li, X, Raraigh, KS, Robbins, S, Han, ST, Franca, AL, Pellicore, MJ, Evans, TA, Arcara, KM, Nguyen, H, Luan, S, Belchis, D, Hertecant, J, Zabner, J, Sly, WS, Cutting, GR (23 February 2016). "Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease". Human Molecular Genetics. 25 (10): 1923-1933. 3. Almståhl, A, Wikström, M (May 2003). "Electrolytes in stimulated whole saliva in individuals with hyposalivation of different origins". Archives of Oral Biology. 48 (5): 337-44. doi:10.1016/s0003-9969(02)00200-5. PMID 12711377. Manokaran, S, Zhang, X, Chen, W, Srivastava, DK (June 2010). "Differential modulation of the active site environment of human carbonic anhydrase XII by cationic quantum dots and polylysine". Biochimica et Biophysica Acta. 1804 (6): 1376-84.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur CA12

Antigène: CA12 (Carbonic Anhydrase 12 (CA12))

Autre désignation: CA12

Sujet:

Synonyms: Carbonic anhydrase 12, Carbonate dehydratase XII, Carbonic anhydrase XII, CA-XII, Tumor antigen HOM-RCC-3.1.3, CA12

Tissue Specificity: Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat glands and bronchiolar epithelium (PubMed:26911677).

Background: Carbonic anhydrase 12 is an enzyme that in humans is encoded by the CA12 gene. It is mapped to 15q22.2. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10 % of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene.

Poids moléculaire: 45 kDa

ID gène: 771

UniProt: O43570